1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
|
#
# The Python Imaging Library.
#
# SPIDER image file handling
#
# History:
# 2004-08-02 Created BB
# 2006-03-02 added save method
# 2006-03-13 added support for stack images
#
# Copyright (c) 2004 by Health Research Inc. (HRI) RENSSELAER, NY 12144.
# Copyright (c) 2004 by William Baxter.
# Copyright (c) 2004 by Secret Labs AB.
# Copyright (c) 2004 by Fredrik Lundh.
#
##
# Image plugin for the Spider image format. This format is is used
# by the SPIDER software, in processing image data from electron
# microscopy and tomography.
##
#
# SpiderImagePlugin.py
#
# The Spider image format is used by SPIDER software, in processing
# image data from electron microscopy and tomography.
#
# Spider home page:
# https://spider.wadsworth.org/spider_doc/spider/docs/spider.html
#
# Details about the Spider image format:
# https://spider.wadsworth.org/spider_doc/spider/docs/image_doc.html
#
from __future__ import print_function
import os
import struct
import sys
from PIL import Image, ImageFile
def isInt(f):
try:
i = int(f)
if f - i == 0:
return 1
else:
return 0
except (ValueError, OverflowError):
return 0
iforms = [1, 3, -11, -12, -21, -22]
# There is no magic number to identify Spider files, so just check a
# series of header locations to see if they have reasonable values.
# Returns no. of bytes in the header, if it is a valid Spider header,
# otherwise returns 0
def isSpiderHeader(t):
h = (99,) + t # add 1 value so can use spider header index start=1
# header values 1,2,5,12,13,22,23 should be integers
for i in [1, 2, 5, 12, 13, 22, 23]:
if not isInt(h[i]):
return 0
# check iform
iform = int(h[5])
if iform not in iforms:
return 0
# check other header values
labrec = int(h[13]) # no. records in file header
labbyt = int(h[22]) # total no. of bytes in header
lenbyt = int(h[23]) # record length in bytes
if labbyt != (labrec * lenbyt):
return 0
# looks like a valid header
return labbyt
def isSpiderImage(filename):
with open(filename, "rb") as fp:
f = fp.read(92) # read 23 * 4 bytes
t = struct.unpack(">23f", f) # try big-endian first
hdrlen = isSpiderHeader(t)
if hdrlen == 0:
t = struct.unpack("<23f", f) # little-endian
hdrlen = isSpiderHeader(t)
return hdrlen
class SpiderImageFile(ImageFile.ImageFile):
format = "SPIDER"
format_description = "Spider 2D image"
_close_exclusive_fp_after_loading = False
def _open(self):
# check header
n = 27 * 4 # read 27 float values
f = self.fp.read(n)
try:
self.bigendian = 1
t = struct.unpack(">27f", f) # try big-endian first
hdrlen = isSpiderHeader(t)
if hdrlen == 0:
self.bigendian = 0
t = struct.unpack("<27f", f) # little-endian
hdrlen = isSpiderHeader(t)
if hdrlen == 0:
raise SyntaxError("not a valid Spider file")
except struct.error:
raise SyntaxError("not a valid Spider file")
h = (99,) + t # add 1 value : spider header index starts at 1
iform = int(h[5])
if iform != 1:
raise SyntaxError("not a Spider 2D image")
self._size = int(h[12]), int(h[2]) # size in pixels (width, height)
self.istack = int(h[24])
self.imgnumber = int(h[27])
if self.istack == 0 and self.imgnumber == 0:
# stk=0, img=0: a regular 2D image
offset = hdrlen
self._nimages = 1
elif self.istack > 0 and self.imgnumber == 0:
# stk>0, img=0: Opening the stack for the first time
self.imgbytes = int(h[12]) * int(h[2]) * 4
self.hdrlen = hdrlen
self._nimages = int(h[26])
# Point to the first image in the stack
offset = hdrlen * 2
self.imgnumber = 1
elif self.istack == 0 and self.imgnumber > 0:
# stk=0, img>0: an image within the stack
offset = hdrlen + self.stkoffset
self.istack = 2 # So Image knows it's still a stack
else:
raise SyntaxError("inconsistent stack header values")
if self.bigendian:
self.rawmode = "F;32BF"
else:
self.rawmode = "F;32F"
self.mode = "F"
self.tile = [("raw", (0, 0) + self.size, offset, (self.rawmode, 0, 1))]
self.__fp = self.fp # FIXME: hack
@property
def n_frames(self):
return self._nimages
@property
def is_animated(self):
return self._nimages > 1
# 1st image index is zero (although SPIDER imgnumber starts at 1)
def tell(self):
if self.imgnumber < 1:
return 0
else:
return self.imgnumber - 1
def seek(self, frame):
if self.istack == 0:
raise EOFError("attempt to seek in a non-stack file")
if not self._seek_check(frame):
return
self.stkoffset = self.hdrlen + frame * (self.hdrlen + self.imgbytes)
self.fp = self.__fp
self.fp.seek(self.stkoffset)
self._open()
# returns a byte image after rescaling to 0..255
def convert2byte(self, depth=255):
(minimum, maximum) = self.getextrema()
m = 1
if maximum != minimum:
m = depth / (maximum - minimum)
b = -m * minimum
return self.point(lambda i, m=m, b=b: i * m + b).convert("L")
# returns a ImageTk.PhotoImage object, after rescaling to 0..255
def tkPhotoImage(self):
from PIL import ImageTk
return ImageTk.PhotoImage(self.convert2byte(), palette=256)
def _close__fp(self):
try:
if self.__fp != self.fp:
self.__fp.close()
except AttributeError:
pass
finally:
self.__fp = None
# --------------------------------------------------------------------
# Image series
# given a list of filenames, return a list of images
def loadImageSeries(filelist=None):
"""create a list of :py:class:`~PIL.Image.Image` objects for use in a montage"""
if filelist is None or len(filelist) < 1:
return
imglist = []
for img in filelist:
if not os.path.exists(img):
print("unable to find %s" % img)
continue
try:
im = Image.open(img).convert2byte()
except Exception:
if not isSpiderImage(img):
print(img + " is not a Spider image file")
continue
im.info["filename"] = img
imglist.append(im)
return imglist
# --------------------------------------------------------------------
# For saving images in Spider format
def makeSpiderHeader(im):
nsam, nrow = im.size
lenbyt = nsam * 4 # There are labrec records in the header
labrec = int(1024 / lenbyt)
if 1024 % lenbyt != 0:
labrec += 1
labbyt = labrec * lenbyt
hdr = []
nvalues = int(labbyt / 4)
for i in range(nvalues):
hdr.append(0.0)
if len(hdr) < 23:
return []
# NB these are Fortran indices
hdr[1] = 1.0 # nslice (=1 for an image)
hdr[2] = float(nrow) # number of rows per slice
hdr[5] = 1.0 # iform for 2D image
hdr[12] = float(nsam) # number of pixels per line
hdr[13] = float(labrec) # number of records in file header
hdr[22] = float(labbyt) # total number of bytes in header
hdr[23] = float(lenbyt) # record length in bytes
# adjust for Fortran indexing
hdr = hdr[1:]
hdr.append(0.0)
# pack binary data into a string
hdrstr = []
for v in hdr:
hdrstr.append(struct.pack("f", v))
return hdrstr
def _save(im, fp, filename):
if im.mode[0] != "F":
im = im.convert("F")
hdr = makeSpiderHeader(im)
if len(hdr) < 256:
raise IOError("Error creating Spider header")
# write the SPIDER header
fp.writelines(hdr)
rawmode = "F;32NF" # 32-bit native floating point
ImageFile._save(im, fp, [("raw", (0, 0) + im.size, 0, (rawmode, 0, 1))])
def _save_spider(im, fp, filename):
# get the filename extension and register it with Image
ext = os.path.splitext(filename)[1]
Image.register_extension(SpiderImageFile.format, ext)
_save(im, fp, filename)
# --------------------------------------------------------------------
Image.register_open(SpiderImageFile.format, SpiderImageFile)
Image.register_save(SpiderImageFile.format, _save_spider)
if __name__ == "__main__":
if len(sys.argv) < 2:
print("Syntax: python SpiderImagePlugin.py [infile] [outfile]")
sys.exit()
filename = sys.argv[1]
if not isSpiderImage(filename):
print("input image must be in Spider format")
sys.exit()
im = Image.open(filename)
print("image: " + str(im))
print("format: " + str(im.format))
print("size: " + str(im.size))
print("mode: " + str(im.mode))
print("max, min: ", end=" ")
print(im.getextrema())
if len(sys.argv) > 2:
outfile = sys.argv[2]
# perform some image operation
im = im.transpose(Image.FLIP_LEFT_RIGHT)
print(
"saving a flipped version of %s as %s "
% (os.path.basename(filename), outfile)
)
im.save(outfile, SpiderImageFile.format)
|